Ágnes Kittel

BIOGRAPHICAL SKETCH
NAME
Kittel, Ágnes
POSITION TITLE
Scientific Advisor
eRA COMMONS
USER NAME
kittel
INSTITUTION AND LOCATION DEGREE
(if applicable)
YEAR(s) FIELD OF STUDY
Eötvös Loránd University, Budapest, Hungary MS 1981 Chemistry
Eötvös Loránd University, Budapest, Hungary DUniv 1988 Biochemistry
Hungarian Academy of Sciences, Budapest, Hungary PhD 1996 Biology
Hungarian Academy of Sciences, Budapest, Hungary DSc 2006 Cell biology

Positions and Employment
1981-1983               Assistant Research Fellow, 2nd Institute of Chemistry-Biochemistry, Semmelweis Medical University, Budapest, Hungary
CHILD-CARE, OUT of  SCIENTIFIC WORK
1988-1989               Assistant research fellow, Institute of Experimental Medicine, Hungarian Academy of Sciences, (IEM  HAS) Budapest, Hungary
CHILD-CARE, OUT of  SCIENTIFIC WORK
1992-1995              Associate research fellow, IEM HAS Budapest, Hungary 
1996-2006                              Senior research fellow, IEM HAS, Budapest, Hungary
2007-present          Scientific advisor, IEM HAS, Budapest, Hungary

Study trips :
1993:                      Visiting scientist for the J. W. Goethe Universität,  Zoologische Institute, Frankfurt, Germany
1995:                                      Visiting scientist for the H.S.Raffaele Scientific Institute, Milan, Italy 
1995-1996:              Visiting scientistfor the Dept. of Neurosciences, UCSD, San Diego, US 
1998:                                      Visiting scientist for the Rega Institute, Leuven, Belgium
Visiting scientist for the Harvard Medical School - Beth Israel Deaconess Medical Center, Boston, USA
1999:                                      Visiting scientist for the LUC,Diepenbeek, Belgium
2000:                                      Visiting scientist  for the IMIM, Barcelona, Spain
Visiting scientist  for the Weizmann Institute of Science, Rehovot, Israel
Visiting scientist for the School of Biological Sciences, Univ. Manchester
2001:                                      Visiting professor for the Harvard Medical School - BIDMC, Boston, USA
2002:                                      Visiting scientist for  the LAVAL University, Quebec, Canada
2005:                                      Visiting scientist for the Nathan Kline Institute, Orangeburg, USA
2008:                                      Visiting scientist for the Department of Pharmacological Sciences, University of Milan
2010:                       Visiting scientist for the Department of Pharmacological Sciences, University of Milan

Tutorial experience:

1981-1983               Lecturer, 2nd Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary

Fields of interest:

Localization and characterization of the players of purinergic signalling in the central nervous system.
Identification of extracellular vesicles of different origin.
Investigation of the blood-brain barrier and comparison the feature of its in vitro models by means of electron microscopy

Professional Activities  

2008-2011                               Member of Committee on Cellular Biology and Phylogenesis, Hungarian Academy of Sciences
2002-present                           secretary of the Hungarian Society for Microscopy
2010-present                           President of the Hungarian Electron Microscopy Foundation
2016 -                                     Executive Board member of  European Society for Microscopy

Membership of Scientific Societies
Hungarian Society of Experimental and Clinical Pharmacology
Hungarian Society of Neuroscience
Federation of European Neuroscience Societies
Hungarian Society for Microscopy
European Society for Microscopy

Other membership

Club of Hungarian Science Journalists
European Union of Science Journalists' Associations

Awards and Main Fellowships:
1995:       Eötvös Hungarian State Fellowship
1998:       Soros Short Term Fellowship
1999:       IBRO Fellowship
2000:       Award of the Hungarian Electron Microscopy Foundation
2000:       FEBS Short Term Fellowship
2002:       2nd Prize of Hungarian Research Fund and “Life and Science” journal

Editorial Board membership

2012-present           Editorial Board member of Applied Cell Biology, Trade Science Ins, Indian S. Press

Ongoing Research Support

Hungarian National Research Foundation (2 grants)

International Collaborations
Past :
Prof. Jacopo Meldolesi and Dr. Fabio Grohovac,H.S.Raffaele Scientific Institute, Milan, Italy 
Prof. Tsunao Saitoh and Dr. Elieser Masliah, UCSD, San Diego
Prof. Luc Vanduffel, UCL, Hasselt, Belgium
Prof. Ghislain Oppdenakker, Rega Institute, Leuven, Belgium
Prof. Mordechai Liscovitch, Weizmann Institute, Rehovot, Israel
Prof. F.X. Real, IMIM, Barcelona, Spain
Prof. Martin Stewart, School of Biological Sciences, Univ. Manchester, UK 
Prof. Dr, Simon C. Robson, Harvard Medical School - BIDMC, Boston, USA
Assoc. Prof. Jean Sevigny, LAVAL University, Quebec, Canada
Dr. Terry Kirley, College of Medicine, University of Cincinnati, Cincinnati, OH, USA
Dr. Henry Sershen, Center for Neurochemistry, Orangeburg, New York, USA
Prof. Dr. Herbert Zimmermann, J. W. Goethe Universität,  Zoologische Institute, Frankfurt, Germany

ongoing
Prof. Dr. Maria Pia Abbracchio, Department of Pharmacological Sciences, University of Milan
Dr. Jochen Klucken, University Hospital Erlangen, Friedrich‐Alexander‐University Erlangen‐Nürnberg, Germany

Conference organisation:
1999  Member of the organizing committee of 2nd International Workshop on Ecto-ATPases”. June 14-18, Diepenbeek, Belgium
2001 Faculty member of the “The Advanced School of Neurochemistry 5th Biennial  Course, August 22-26,  Buenos Aires, Argentina
2004 Faculty member of the “The Advanced School of Neurochemistry 6th Biennial   Course”, May 10-13, Avignon, France
2004-2005 Member of the organizing committee of Multinational Conference for Microscopy  2005, Portoroz, Slovenia
2008-2009 Member of the organizing committee of Multinational Conference for Microscopy  2009, Graz, Austria
2010-2011 Member of the scientific committee of Multinational Conference for Microscopy  2011, Urbino, Italy
2011-2012 Member of the scientific committee of European Microscopy Congress  2012, Manchester, UK
2012-2013 Member of the scientific committee of Multinational Conference for Microscopy 2013, Regensburg, Germany
2002- present Organizer of the annual meetings  of the Hungarian Society for Microscopy
2015 Chair of the Multinational Conference for Microscopy 2015, Eger, Hungary

C. Selected papers published in EV field

  1. Stefania Ceruti, Laura Colombo, Giulia Magni, Francesca Viganò, Marta Boccazzi, Mária A. Deli, Beáta Sperlágh, Maria P. Abbracchio, Ágnes Kittel  (2011) Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrier. Neurochemistry International 59 (2011) 259–271
  2. Bence György,Tamás G Szabó, Mária Pásztói, Zsuzsanna Pál,Petra Misják, BorbálaAradi, Valéria László, Éva Pállinger, Erna Pap, Ágnes Kittel, György Nagy, András Falus, Edit Buzás (2011) Membrane vesicles, current state-of-the-art: Emerging role of the extracellular vesicles. Cell Mol Life Sci 68: 2667-2688
  3. Gyorgy B, Modos K, Pallinger E, Kittel A and Buzás EI. (2011) Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 117:e39-48.
  4. Bence György, Tamás G Szabó, Lilla Turiák, Matthew Wright, Petra Herczeg, Zsigmond Lédeczi, Ágnes Kittel, Anna Polgár, Kálmán Tóth, Beáta Dérfalvi, Gergő Zelenák, István Böröcz, Bob Carr, György Nagy, Károly Vékey, Steffen Gay, András Falus, Edit I Buzás (2012) Improved flow cytometric assessment reveals distinct microvesicle (cell-derived microparticle) signatures in joint diseases. Plos One, 7(11) e49726
  5. Maria Pasztoi, Barbara Sodar, Petra Misjak, Krisztina Paloczi, Agnes Kittel, Kalman Toth, Karoly Wellinger, Pal Geher, Gyorgy Nagy, Tamas Lakatos, Andras Falus, Edit Iren Buzas (2013) The recently identified hexosaminidase D enzyme substantially contributes to the elevated hexosaminidase activity in rheumatoid arthritis. Immunology Letters 149 (2013) 71– 76
  6. Csaba I Timár, Ákos M Lőrincz, Roland Csépányi-Kömi, Anna Vályi-Nagy, Gyorgy Nagy, Edit Buzas, Zsolt Iványi, Ágnes Kittel, David W Powell, Kenneth R McLeish, and Erzsebet Ligeti (2013) Antibacterial effect of microvesicles released from human neutrophilic granulocytes. Blood, 121(3) 510-518.
  7. Kittel A., A. Falus, E. Buzás (2013) Microencapsulation technology by nature: cell derived extracellular vesicles with therapeutic potential European Journal of Microbiology and Immunology 3 (2013) 2, pp. 91–96.  DOI: 10.1556/EuJMI.3.2013.2.1  
  8. Zoltán Giricz,Zoltán V Varga,Tamás Baranyai,Péter Sipos,Krisztina Pálóczi, Agnes Kittel,Edit Buzás,Péter Ferdinandy Cardioprotection by remote ischemic preconditioning of the rat heart is mediated by extracellular vesicles. Journal of Molecular and Cellular CardiologyDOI:10.1016/j.yjmcc.2014.01.004
  9. Géza Tamás Szabó, Bettina Tarr, Krisztina Pálóczi, Katalin Éder, Eszter Lajkó, Ágnes Kittel, Sára Tóth, Bence György, Mária Pásztói, Andrea Németh, Xabier Osteikoetxea, Éva Pállinger, András Falus, Katalin Szabo‑Taylor, Edit Irén Buzás (2014) Critical role of extracellular vesicles in modulating the cellular effects of cytokines. Cell. Mol. Life Sci. DOI 10.1007/s00018-014-1618-z
  10. Ákos M Lőrincz, Csaba I Timár, Krisztina A Marosvári, Dániel S Veres, Lilla Otrokocsi, Ágnes Kittel, Erzsébet Ligeti (2014) Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes. Journal of Extracellular Vesicles. 2014/12/22
  11. Xabier Osteikoetxea, Andrea Balogh, Katalin Szabó-Taylor, Andrea Németh, Tamás Géza Szabó, Krisztina Pálóczi, Barbara  Sódar, Ágnes Kittel, Bence György, Éva Pállinger, János Matkó, Edit Irén Buzás(2015) Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties. Plos One DOI: 10.1371/journal.pone.0121184
  12. Ákos M. Lőrincz, Mária Schütte, Csaba I. Timár, Daniel S. Veres, Ágnes Kittel, Kenneth R. McLeish, Michael L. Merchant, Erzsébet Ligeti (2015) Functionally and morphologically distinct populations of extracellular vesicles produced by human neutrophilic granulocytes,  J Leukoc Biol, 98: 583-9 doi:10.1189/jlb.3VMA1014-514R
  13. Xabier Osteikoetxea , Barbara Sódar , Andrea Németh, Katalin Szabó-Taylor, Krisztina Pálóczi, Krisztina V Vukman, Viola Tamási, Andrea Balogh, Ágnes Kittel, Éva Pállinger, Edit Irén Buzás (2015)  Differential detergent sensitivity of extracellular vesicle subpopulations. Organic & Biomolecular Chemistry DOI: 10.1039/C5OB01451D 
  14. Petra Sántha, Szilvia Veszelka ,Zsófia Hoyk, Mária Mészáros, Fruzsina R. Walter, Andrea E. Tóth,Lóránd  Kiss,András, Kincses,Zita Oláh,György Seprényi,Gabor Rakhely,András Dér,Magdolna Pákáski,Janos Kalman, Ágnes Kittel*, Maria A Deli*: Restraint stress-induced morphological changes at the blood-brain barrier in adult rats. Front. Mol. Neurosci. | doi: 10.3389/fnmol.2015.00088
  15. Tamás Baranyai, Kata Herczeg, Zsófia Onódi, István Voszka, Károly Módos, Nikolett Marton, György Nagy, Imre Mäger, Matthew J Wood, Samir El-Andaloussi, Zoltán Pálinkás, Vikas Kumar, Péter Nagy, Ágnes Kittel, Edit Irén Buzás, Péter Ferdinandy, and Zoltán Giricz: Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods. PLoS One.2015 Dec 21;10(12):e0145686. doi: 10.1371/journal.pone.0145686. eCollection 2015.
  16. Barbara Sodar, Agnes Kittel, Krisztina Paloczi, Krisztina Vukman, Xabier Osteikoetxea, Katalin Szabó-Taylor, Andrea Nemeth, Beáta Sperlágh, Tamas Baranyai, Zoltan Giricz, Zoltan Wiener, Lilla Turiak, Laszlo Drahos, Eva Pallinger, Karoly Vekey, Peter Ferdinandy, Andras Falus, and Edit Buzás Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection Scientific Reports 6:24316·April 2016 DOI: 10.1038/srep24316